Our laboratory is primarily interested in understanding the molecular mechanisms of signal transduction and physiological responses that allow cells to adapt to stress conditions. Our main study model is Trypanosoma cruzi, a unicellular eukaryote responsible for a major health problem in Latin America, Chagas disease. The advantage of using T. cruzi as an experimental model lies mainly in the fact that this organism presents a complex life cycle during which it must cope with sudden changes in the environment to which it must respond immediately to survive. In addition, taking into account that the trypanosomatids are a group of evolutionarily ancient organisms, they provide a distant reference point that allows the dissection of how crucial biological processes evolved in this and other taxonomic groups.

 

In the lab, we mainly use biochemistry, bioinformatics, genetics, molecular biology and cellular biology techniques to elucidate how the trypanosomatids respond to stressors, survive and differentiate along their life cycle. In this way, we seek to expose new therapeutic targets to combat Chagas disease and other trypanosomiasis.

 

Lab Members

Alejandra Cecilia Schoijet
Investigador Asistente CONICET
Ayudante de 1ra FCEN UBA
aschoijet@gmail.com

Milena Massimino Stepñicka
Estudiante de Doctorado
Beca Agencia

Tamara Sternlieb
Becaria Conicet
tamara.sternlieb@gmail.com

Nadia Barrera.
Becaria FONCyT

Patricio Genta.
Estudiante de Grado

Publications

1) Defining the role of a FYVE domain in the localization and activity of a cAMP
phosphodiesterase implicated in osmoregulation in Trypanosoma cruzi.
Schoijet AC, Miranda K, Medeiros LC, de Souza W, Flawiá MM, Torres HN, Pignataro OP, Docampo R, Alonso GD.
Mol Microbiol. 2011 Jan;79(1):50-62. doi: 10.1111/j.1365-2958.2010.07429.x. Epub 2010 Oct 28.

2) A Trypanosoma cruzi phosphatidylinositol 3-kinase (TcVps34) is involved in osmoregulation and receptor-mediated endocytosis.
Schoijet AC, Miranda K, Girard-Dias W, de Souza W, Flawiá MM, Torres HN, Docampo R, Alonso GD.
J Biol Chem. 2008 Nov 14;283(46):31541-50. doi: 10.1074/jbc.M801367200. Epub 2008 Sep 17.

3) TcrPDEA1, a cAMP-specific phosphodiesterase with atypical pharmacological properties from Trypanosoma cruzi.
Alonso GD, Schoijet AC, Torres HN, Flawiá MM.
Mol Biochem Parasitol. 2007 Mar;152(1):72-9. Epub 2006 Dec 29.

4) TcPDE4, a novel membrane-associated cAMP-specific phosphodiesterase from Trypanosoma cruzi.
Alonso GD, Schoijet AC, Torres HN, Flawiá MM.
Mol Biochem Parasitol. 2006 Jan;145(1):40-9. Epub 2005 Sep 30.

5) Arginine kinase: a common feature for management of energy reserves in African and American flagellated trypanosomatids.
Pereira CA, Alonso GD, Torres HN, Flawiá MM.
J Eukaryot Microbiol. 2002 Jan-Feb;49(1):82-5.

6) Trypanosoma cruzi arginine kinase characterization and cloning. A novel energetic pathway in protozoan parasites.
Pereira CA, Alonso GD, Paveto MC, Iribarren A, Cabanas ML, Torres HN, Flawiá MM.
J Biol Chem. 2000 Jan 14;275(2):1495-501.

Grants

1) Proyectos de Investigación Plurianuales (PIP) 2010-2012. CONICET. Nro. 00057.
“Mecanismos de Transducción de Señales y de Regulación de la Expresión Génica en Trypanosoma cruzi”

2) Proyectos de Investigación Científica y Tecnológica 2010 (PICT Bicentenario). 2011-2014. ANPCyT, FONCyT. Nro. 00199.
“Mecanismos regulatorios involucrados en la respuesta a diferentes tipos de estrés en Trypanosoma cruzi”

3) UBACyT 2013-2016. Universidad de Buenos Aires. Nro. 00141.
“Estudio de proteínas involucradas en la regulación del ciclo celular y la diferenciación entre estadios en Trypanosoma cruzi”

4) UBACyT 2011-2014. Universidad de Buenos Aires. Nro. 00015.
“Mecanismos de transducción de señales determinantes de la respuesta adaptativa en T. cruzi”

Ex Lab Members

Dr. Matias Fassolari
mfassolari@gmail.com

Ph.D. Guillermo Daniel Alonso

galonso@dna.uba.ar
buiyimail@gmail.com

  • 1996. Licenciado en Ciencias Biológicas.
  • 2002. Doctor de la Universidad de Buenos Aires, Área Ciencias Químicas. Facultad de Ciencias Exactas y Naturales. Argentina.
  • 2006. “Postdoctoral Visiting Fellow in the NIH Visiting Program” en el laboratorio del Dr. Lutz Birnbaumer, en el “National Institute of Environmental Health Sciences”, North Carolina, U.S.A.
  • 2014-actualidad. Investigador Independiente, CONICET. Argentina.
  • 2015-actualidad. Profesor Adjunto. Simple, Regular. Departamento de Fisiología, Biología Molecular y Celular. Área Biología Molecular y Celular. FCEyN UBA.
  • 2015-2016. Tesorero de la Sociedad Argentina de Protozoología y Enfermedades Parasitarias. Argentina.